Uphill energy transfer in LH2-containing purple bacteria at room temperature
نویسندگان
چکیده
Uphill energy transfer in the LH2-containing purple bacteria Rhodopseudomonas acidophila, Rhodopseudomonas palustris, Rhodobacter sphaeroides, Chromatium vinosum and Chromatium purpuratum was studied by stationary fluorescence spectroscopy at room temperature upon selective excitation of the B800 pigments of LH2 and the B880 pigments of LH1 at 803 nm and 900 nm, respectively. The resulting fluorescence spectra differed significantly at wavelengths shorter than the fluorescence maximum but agreed at longer wavelengths. The absorption spectra of the species studied were decomposed into five bands at approx. 800, 820, 830, 850 and 880 nm using the shapes of the absorption spectra of the LH1-RC only species Rhodospirillum rubrum and the isolated B800-850 complex from Rps. acidophila strain 10050 as guide spectra. This allowed a quantification of the number of pigments in each pigment group and, consequently, the antenna size of the photosynthetic unit assuming 36 bacteriochlorophyll a molecules in an LH1-RC complex. In most of the LH2-containing purple bacterial strains the number of LH2 rings per LH1-RC was less than the idealized number of eight (Papiz et al., Trends Plant Sci. 1 (1996) 198-206), which was achieved only by C. purpuratum. Uphill energy transfer was assayed by comparing the theoretical fluorescence spectrum obtained from a Boltzmann equilibrium with the measured fluorescence spectrum obtained by 900 nm excitation. The good match of both spectra in all the purple bacteria studied indicates that uphill energy transfer occurs practically up to its thermodynamically maximal possible extent. All strains studied contained a small fraction of either poorly connected or unconnected LH2 complexes as indicated by higher fluorescence yields from the peripheral complexes than predicted by thermal equilibration or kinetic modeling. This impedes generally the quantitative analysis of blue-excited fluorescence spectra.
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عنوان ژورنال:
- Biochimica et biophysica acta
دوره 1412 2 شماره
صفحات -
تاریخ انتشار 1999